Cataloguenumber
CYT-1111
Synonyms
23-219aa(IL12A)/23-328aa(IL12B),Interleukin12(subunitbeta/alpha),IL12His,NKSF1,NKSF,CTLmaturationfactor(TCMF),Cytotoxiclymphocytematurationfactor(CLMF),TSF,Edodekin-alpha,IL-12.
Introduction
interleukin12subunitbeta/alphaorIL12b/IL12aisagrowthfactorcytokinewhichincreasesthelyticactivityofNK/lymphokine-activatedkillercells,activatedTandNKcellsandpromptIFN-gammaproduction(throughrestingPBMC).IL12b/IL12aisanessentialparttotheprocessofcellular-immunityandactivatesthedifferentiationofTh1cellsoriginatesfromtheprecursorThelpercells.TheproteinislinkedtoIL23AandcreatestheIL-23interleukin,bythat,theautoimmuneinflammationisinducedandautoimmuneinflammatorydiseasesandtumOrigenesisarebeingaffected.
Description
Interleukin12HumanRecombinantisaglycosylatedheterodimercontaining503aminoacids(306aminoacidrHuIL-12p40andthe197aminoacidrHuIL-12p35subunits)andhavingamolecularmassof60kDa.IL12ispurifiedbyproprietarychromatographictechniques.
Source
Baculovirus.
PhysicalAppearance
Formulation
Lyophilizedfroma0.2µmfilteredconcentratedsolutioninPBS,pH7.2.
Solubility
ItisrecommendedtoreconstitutethelyophilizedInterleukin12insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedInterleukin12althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionPDGFBBshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedbyanalysisbySDS-PAGE.
Aminoacidsequence
IL-12isaheterodimerofIL-12AandIL-12Blinkedthroughadisulfide-bond:
>IL-12ARNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS.
>IL-12B
IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS.
BIOLOGicalActivity
DeterminedbycellproliferationassayusingPHA-stimulatedhumanTlymphoblasts.TheED50forthiseffectis0.01-0.05ng/mLcorrespondingtoaspecificactivityof1.1×104units/μg,whichiscalibratedagainstrHuIL-12WHOStandard.
SafetyDataSheet
SDS
