Catalogue number
CYT-050
Synonyms
Interleukin 23 alpha subunit p19, Interleukin-12 subunit beta p40, SGRF, IL23P19, IL-23-A, interleukin-six, G-CSF related factor, JKA3 induced upon T-cell activation, interleukin 12B (natural killer cell stimulatory factor 2 cytotoxic lymphocyte maturation factor 2 p40), NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF2, CLMF p40.
Introduction
IL23 is composed of a subunit of the heterodimeric cytokine IL23 and the p40 subunit of interleukin 12 (IL12B). Interleukin-23 (IL-23) belongs to the IL-12 family and is produced by antigen presenting cells. IL-23 using IL12RB1 and IL-23R (specific for IL-23) can activate STAT and NF-kB pathways and stimulate the production of IFN-gamma. ). IL-23 is known to take a vital part in the inflammation process and is associated with auto immune diseases. However, unlike IL12, which acts primarily on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.
Description
IL-23 Human Recombinant produced in HEK cells is a 55kDa heterodimeric protein composed of 2 disulfide-linked subunits - 19kDa (p19) and 43 kDa (p40).
Source
HEK.
Physical Appearance
IL-23 if supplied as a sterile filtered White lyophilized (freeze-dried) powder.
Formulation
IL-23 was lyophilized from a 0.1µm filtered solution (1.05mg/ml) containing 1xPBS.
Solubility
It is recommended to reconstitute the lyophilized IL23 in sterile 1xPBS containing 0.1% endotoxin-free recombinant HSA.
Stability
Lyophilized Interleukin-23 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-23 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by analysis by SDS-PAGE.
Biological Activity
The activity, as determined by the dose dependent secretion of IL-17 in response to IL-23 using murine splenocytes, the ED50 is 1.9ng/ml.
Safety Data Sheet
Usage
ProSpec"s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
