Cataloguenumber
CYT-382
Synonyms
T-cellgrowthfactor(TCGF),Aldesleukin,Lymphokine,IL-2.
Introduction
Description
Interleukin-2RatRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining135aminoacidsandhavingamolecularmassof16kDa.
TheIL-2ispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
TheIL-2solutioncontains0.2µmfilteredsolutionin20mMPBpH3.5and30%Glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedbySDS-PAGEandHPLC.
Aminoacidsequence
APTSSPAKETQQHLEQLLLDLQVLLRGIDNYKNLKLPMMLTFKFYLPKQATELKHLQCLENELGALQRVLDLTQSKSFHLEDAGNFISNIRVTVVKLKGSENKFECQFDDEPATVVEFLRRWIAICQSIISTMTQ.
BIOLOGicalActivity
TheED50asdeterminedbythedose-dependantstimulationofmurineCTLL-2cellsislessthan0.2ng/ml,correspondingtoaSpecificActivityof5,000,000IU/mg.
References
SafetyDataSheet
SDS

