
Cataloguenumber
CYT-1117
Synonyms
Interferonalpha-2,IFN-alpha-2,Interferonalpha-A,LeIFA,IFNA2,IFNA2A,IFNA2B,IFNA2C.
Introduction
IFN-alphaisproducedbymacrophagesandhasantiviralactivities.IFNstimulatestheproductionoftwoenzymes:proteinkinaseandanoligoadenylatesynthetase.
Description
Interferon-alpha2cRecombinantHumanproducedinyeastisasingle,glycosylatedpolypeptidechaincontaining165aminoacidsandhavingamolecularmassof19.3kDa.TheIFNA2Cispurifiedbyproprietarychromatographictechniques.
Source
Saccharomycescerevisiae.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutioninPBS,pH7.4and0.02%Tween-20.
Solubility
ItisrecommendedtoreconstitutethelyophilizedInterferon-alpha2cinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedIFNA2Calthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionInterferon-alpha2cshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
CDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRRDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE.
BIOLOGicalActivity
Theactivityisdeterminedbythecytopathiceffectinhibitionassay.
SafetyDataSheet
SDS