
Cataloguenumber
CYT-1036
Synonyms
IL-27B,IL27B,IL27-B,EBI-3,Interleukin-27beta,IL-27subunitbeta,Epstein-Barrvirus-inducedgene3proteinhomolog,EBI3.
Introduction
EBI3hasaninducedexpressioninBlymphocytesinreactiontoEpstein-Barrvirusinfection.EBI3encodesasecretedglycoproteinbelongingtothehematopoietinreceptorfamily,andheterodimerizeswitha28kDaproteintoformiIL-27.EBI3drivesrapidclonalexpansionofnaivecd4(+)t-cells.EBI3stronglysynergizeswithIL-12toactivateIFN-gammaproductionofnaivecd4(+)t-cells.EBI3mediatesitsBIOLOGiceffectsthroughthecytokinereceptorwsx-1/tccr.
Description
EBI3MacaqueRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining210aminoacidsandhavingamolecularmassof23.4kDa.TheEBI3ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
Lyophilizedfromasterilefilteredsolutioncontaining0.1%trifluoroaceticacid(TFA).
Solubility
ItisrecommendedtoreconstitutethelyophilizedEBI3insterile18MΩ-cmH2Oataconcentrationof100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedEBI3althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionEBI3Macaqueshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Aminoacidsequence
MRKGPPAALTLPRVQCRAPRYPIAVDCSWTLPPAPNSTSPVSFIATYRFGMAARGHSWPCLQQTPASTSCTIADVRLFSMAPYVLNVTAVHPWGSSSSFVPFIAEHIIKPDPPEGVRLSPLAERQLQVQWEPPRSWPFPEIFSLKYWIRYKRQGAARFHQVGPIEATSFILRAVRPRARYCVQVAAQDLTDYGELSDWSLPATTPMSPGK.
SafetyDataSheet
SDS