Cataloguenumber
CYT-249
Synonyms
ANGPTL4,NL2,ARP4,FIAF,PGAR,HFARP,pp1158,ANGPTL2,Fasting-Induced
ADIposeFactor,HepaticFibrinogen/Angiopoietin-RelatedProtein,PPARGAngiopoietin-RelatedProtein.
Introduction
FIAF(fasting-inducedadiposefactor)a.k.aANGPTL4orPGARorHFARPisanadipocytokineup-regulatedbyfasting,byperoxisomeproliferator-activatedreceptoragoNISTs,andbyhypoxia.ANGPTL4isfoundinhumanandmousebloodplasmabothasanativeproteinandinatruncatedform.InhumanwhiteadiposetissueandSGBSadipocytes,onlythenativeformofANGPTL4couldbedetected,whereasinmicethedifferentiationofmouse3T3-L1adipocytesisassociatedwiththeproductionoftruncatedANGPTL4.However,thetruncatedANGPTL4isproducedbyhumanliver.InhumanbloodplasmaFIAFismainlypresentedinatruncatedform(FIAF-S2),whoselevelstreatmentincreases(asshownbyexperimentaldata).ThereisaninterindividualvariationinANGPTL4levelsofboththetruncatedandthenativeform,howeverthoselevelswerenotinfluencedbyprolongedsemistarvationandarenotassociatedwithbodymassindex.
Description
TheANGPTL4HumanRecombinantismanufacturedwithN-terminalfusionofHisTag.TheAngiopoietin-likeProtein4His-TaggedFusionProteinis25kDaproteincontaining204aminoacidresiduesoftheAngiopoietin-likeProtein4and16additionalaminoacidresidues-HisTag(underlined).
Source
EscherichiaColi.
Purity
Greaterthan95%asdeterminedbySDS-PAGE.
PhysicalAppearance
FilteredWhitelyophilized(freeze-dried)powder.
Formulation
Filteredandlyophilizedfrom0.5mg/mlin0.05MAcetatebufferpH-4.
Solubility
Add0.2mlof0.1MAcetatebufferpH4andletthelyophilizedpelletdissolvecompletely.ForconversionintohigherpHvalue,werecommendintensivedilutionbyrelevantbuffertoaconcentrationof10μg/ml.Inhigherconcentrationsthesolubilityofthisantigenislimited.
StorelyophilizedAngiopoietin-likeProtein4Humanrecombinantat-20°C.AliquottheANGPTL4afterreconstitutiontoavoidrepeatedfreezing/thawingcycles.ReconstitutedANGPTL4canbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C.
Aminoacidsequence
MRGSHHHHHHGMASHMGPVQSKSPRFASWDEMNVLAHGLLQLGQGLREHAERTRSQLSALERRLSACGSACQGTEGSTDLPLAPESRVDPEVLHSLQTQLKAQNSRIQQLFHKVAQQQRHLEKQHLRIQHLQSQFGLLDHKHLDHEVAKPARRKRLPEMAQPVDPAHNVSRLHRLPRDCQELFQVGERQSGLFEIQPQGSPPFLVNCKMTSDGGWTVIQR.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.